"They need to see some guidelines put down in writing so that they know exactly what's happening if they step out of line," said the manager.
"Nobody's wanting them fined. But they have to learn that this can't go on."
Kilmarnock recently introduced a new disciplinary system before Jefferies left Hearts' SPL rivals and he is keen to stamp out the kind of ill discipline that has led to the Edinburgh outfit being fined £50,000 in the past year.
Retaliation gets you in trouble. That's something that we need to address
Hearts manager Jim Jefferies
"This club has been fined heavily for their conduct on the park and that has to change," said Jefferies, who plans to meet managing director Campbell Ogilvie on Monday to discuss the problem. "The fines will only go up and up.
"If it's for dissent and reckless challenges and arguing with the referee, every club would put up a strict code of conduct.
"I've ended up being without Michael Stewart a couple of times for stupid things.
"Christian Nade - before I came here - kicked the ball away and got himself sent off."
Hearts on Sunday entertain Hamilton Accies, who were the opponents when the Edinburgh side had four players and a masseur sent off in a New Douglas Park contest marred by an ugly post-match tunnel brawl.
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Jefferies, who returned to Hearts for a second spell in charge last month, will not tolerate a repeat of such scenes.
Hearts have yet to see red in his four matches back at the helm, but the manager admits that is more by accident than design.
"These things shouldn't happen," he said. "They want to do their battling on the pitch - and I don't mean that in a punch-up way.
"If it's not going your way and you're angry, you've just got to bite your tongue.
"Retaliation gets you in trouble. That's something that we need to address."
Source: BBC Sport